2Cell Biology

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CYTOLOGY AND CELL BIOLOGY

Table 1: Cytology vs Cell Biology

Feature
Cytology
Cell Biology / Modern Cytology
Study includes
Cell organelles + cell inclusions only
Cell organelles + cell inclusions + physiology + chemistry + genetics of cells
Main focus
Form + structure of cell
Structure + function of cell
Father
Robert Hooke
Swanson
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HISTORY OF CELL BIOLOGY

Table 1: Scientists and Contributions

Scientist
Contribution
Robert Hooke
Coined term cell in 1665
Robert Hooke
Observed cork from outer bark of Quercus suber
Robert Hooke
Actually observed dead attached cell walls
Robert Hooke
Published findings in Micrographia
A.V. Leeuwenhoek
Father of microscopy
A.V. Leeuwenhoek
Discovered living cells and free cells
Schleiden
German botanist; proposed cell theory with Schwann
Schwann
German zoologist; proposed cell theory with Schleiden
Dutrochet
First idea of cell theory; separated cells from Mimosa pudica leaf
Rudolf Virchow
Extended cell theory by law of cell lineage
Haberlandt
First idea of cellular totipotency
Steward
Experimentally proved cellular totipotency
Purkinje
Coined term protoplasm
Huxley
Defined protoplasm as physical basis of life
Robert Brown
Discovered nucleus from orchid root cells
Hammerling
Proved nucleus as control centre using Acetabularia
Fontana
Discovered nucleolus
Bowman
Gave term nucleolus
Flemming
Gave term chromatin
Waldeyer
Gave term chromosome
Sutton and Boveri
Proposed chromosome theory of inheritance
Palade
Discovered and named ribosomes
Kolliker
Discovered mitochondria in striated muscle of insects
Benda
Used term mitochondria
Altman
Called mitochondria bioplast
Haeckel
Discovered / gave term plastid
Sachs
Discovered chloroplast
Schimper
Named chloroplast
Porter
First observed endoplasmic reticulum
Camillo Golgi
Discovered Golgi body in nerve cell of cat and barn owl
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CELL THEORY
Classical Cell Theory:
  • All living beings consist of cells
  • Cell = smallest structural + functional unit of life
Cell Lineage:
  • Proposed by Rudolf Virchow
  • Statement: omnis cellula e cellula
  • Meaning: all cells arise from pre-existing cells only by reproduction
Modern / Modified Cell Theory: Cell theory + cell lineage
Synonyms:
  • Cell principle
  • Cell doctrine
  • Modern cell theory
  • Modified cell theory
Exceptions:

Table 1: Exceptions of Cell Theory

Exception
Reason
Virus
Obligate parasite; sub-cellular; major exception
Vaucheria
Multinucleated alga
Rhizopus
Multinucleated fungus
Mucor
Multinucleated fungus
Paramecium
Binucleate / multinucleated protist
Opalina
Multinucleated protist
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CELL SIZE
Normal Cell Size: 0.2 μm to 20 μm
Measurement Units:

Table 1: Units

Microscope
Unit
Light microscope
Micron / μm
Electron microscope
Angstrom / Å
Factors Affecting Cell Size:
  • Cell size ∝ chromosome number
  • Cell size ∝ size of chromosome
  • Cell size ∝ 1 / metabolic activity
  • Cell size ∝ 1 / nucleocytoplasmic ratio
  • Cell size ∝ 1 / surface area per unit volume
Reasoning:
  • Smaller cells are metabolically more active
  • Smaller cells have greater surface area per unit volume
  • Nucleocytoplasmic ratio is directly related to metabolic activity
Smallest Cells:

Table 1: Smallest Cell Facts

Category
Answer
Smallest cell
Mycoplasma gallisepticum / PPLO
PPLO full form
Pleuro pneumonia like organism
Mycoplasma nickname
Joker of plant kingdom
Mycoplasma feature
Without cell wall
Smallest cell with cell wall
Bacterium Dialister pneumosintes
Smallest animal cell
Human sperm cell
Mycoplasma Plant Diseases:
  • Bunchy top of papaya
  • Little leaf of brinjal
  • Clover dwarf
  • Mulberry dwarf
Largest and Longest Cells:

Table 1: Largest / Longest Cell Facts

Category
Answer
Largest prokaryotic cell
Spirillum volutans
Largest animal cell
Ostrich egg cell
Longest animal cell
Nerve cell
Largest plant cell
Acetabularia
Longest plant cell
Fibre of Ramie / Boehmeria nivea
Acetabularia:
  • Unicellular
  • Uninucleate
  • Green alga
  • Umbrella-shaped alga
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CELL DIVIDING CAPACITY
Cellular Totipotency:
Definition: Capacity of a single nucleated living vegetative plant cell to regenerate whole plant
Word Meaning: Totus = all; potens = power
Important Scientists:
  • Idea first given by Haberlandt
  • Experimentally proved by Steward and co-workers
Steward Experiment:
  • Used phloem tissue from carrot root as explant
  • Used coconut milk as liquid medium
  • Coconut milk contains cytokinin
  • Cytokinin induces cell division
Coconut Milk Factor:
  • Cytokinin / zeatin / kinetin
  • Rich in coconut milk
  • Also rich in immature maize grain
Pluripotency:
Definition: Animal cell ability to develop into any type of body cell but not whole animal
Important: Stem cells are totipotent in animals
Cell Ghosts:
  • Empty erythrocytes
  • Difficult to observe under light microscope due to absence of contents
  • When blood is placed in water, erythrocyte contents come out
  • Rupture of RBC = haemolysis
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CELL TYPES BASED ON NUCLEUS

Table 1: Types

Type
Meaning / Feature
Examples
Akaryotic cell
Denucleated cell
Mature mammalian RBC, sieve tube cell
Prokaryotic cell
Nucleoid; no true nucleus
Bacteria, cyanobacteria, archaebacteria, mycoplasma, chlamydia, rickettsia
Mesokaryotic cell
Nuclear membrane present; histone protein absent; nucleolus absent; division by dinomitosis
Dinoflagellates
Eukaryotic cell
True nucleus with nuclear membrane, nucleoplasm and nucleolus
Fungi, protists, Euglena, plants, animals
Prokaryotic vs Eukaryotic:

Table 1: Prokaryotic Cell vs Eukaryotic Cell

Feature
Prokaryotic Cell
Eukaryotic Cell
Nucleus
False nucleus / incipient nucleus / prokaryon / genophore / nucleoid
True well-organised nucleus
Nuclear membrane
Absent
Present
Nucleoplasm
Absent
Present
Nucleolus
Absent
Present
DNA location
Cytoplasm
Nucleoplasm
DNA type
Double-stranded circular DNA
Double-stranded linear DNA
DNA repetition
Non-repetitive
Repetitive
Histone protein
Absent
Present
DNA folding
With RNA + polyamines
With histone proteins
DNA:RNA ratio
1:2
1:1
A+T / G+C ratio
0.88; G+C higher
1.52; G+C lower
Cell wall
Non-cellulosic; peptidoglycan / murein + lipids
Cellulose or chitin
Respiration site
Mesosome / cell membrane
Mitochondria
Membrane-bound organelles
Absent
Present
Envelope system
Single envelope system
Double envelope system
Ribosome
70S
Both 70S and 80S; 80S main
Microtubules + spindle fibres
Absent
Present
Cilia / flagella
Single-stranded; flagellin protein
11-stranded; 2+9 arrangement; tubulin protein
Cell division
Amitosis common; mitosis and meiosis absent
Mitosis + meiosis present; amitosis rare in yeast
Examples
Monera, bacteria, cyanobacteria, archaebacteria, mycoplasma, chlamydia, rickettsia
Fungi, protists, Euglena, plants, animals
Special Points:
  • Most extensively studied prokaryote = E. coli
  • Chlamydia and Rickettsia have both DNA and RNA
  • Chlamydia and Rickettsia = connecting link between virus and bacteria
  • Eukaryotic cells differ from prokaryotic cells by presence of cistrons
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PLANT CELL VS ANIMAL CELL

Table 1: Plant Cell vs Animal Cell

Feature
Plant Cell
Animal Cell
Cell wall
Present; main differentiating feature
Absent
Plastids
Present
Absent
Central vacuole
Large central vacuole
Usually small / absent
Centriole
Usually absent
Present
Lysosome
Usually absent
Present
Mitosis / meiosis
Anastral type
Astral / amphiastral type
Inorganic crystals
Common in cytoplasm
Not common
Special organelles
Sphaerosome, glyoxysome, lomasome
Absent
Cytokinesis
By cell plate
By furrowing
Reserve food
Starch
Glycogen
Hypotonic solution
Does not burst due to cell wall
RBC bursts because cell wall absent
Totipotency
All nucleated cells are totipotent
Fertilized egg + stem cells in blastocyst are totipotent
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CELL WALL
Basic Features:
  • Common in plants
  • Also present in bacteria and cyanobacteria
  • Non-living
  • Porous
  • Permeable
  • Inert
  • Hydrophilic
  • Semitransparent
  • Protective covering secreted by cytoplasm
Constituents:

Table 1: Cell Wall Constituents

Organism / Cell
Cell Wall Material
Plant
Cellulose + hemicellulose + pectin
Bacteria and cyanobacteria
Murein / mucopeptide / peptidoglycan
Fungi
Chitin / fungal cellulose

Table 2: Important Monomers

Substance
Monomer / Components
Cellulose
β-D glucose
Hemicellulose
Arabinose, mannose, xylose, galactose etc.
Pectin
Galactose, galacturonic acid, arabinose
Peptidoglycan polysaccharide
NAG + NAM
Chitin
Polymer of N-acetyl glucosamine / NAG
Plant Cells Without Cell Wall:
  • Mycoplasma
  • Gametes
  • Zoospores
  • Slime moulds / Myxomycetes
Formation:
  • Cell wall forms during cytokinesis
  • Vesicles of ER + Golgi bodies participate
  • Golgi vesicles + spindle proteins at equator = phragmoplast
  • Phragmoplast → cell plate → cell wall
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LAYERS OF CELL WALL

Table 1: Cell Wall Layers

Layer
Position
Nature / Composition
Important Points
Middle lamella
Outermost / between two adjacent cells
Mainly calcium pectate + magnesium pectate
Cementing layer; joins adjacent cells
Primary cell wall
Inner to middle lamella
Cellulose + hemicellulose + pectin in roughly equal amount
Elastic; universal in cells with wall; common in parenchyma + meristematic cells
Secondary cell wall
Inner to primary wall
High cellulose + hemicellulose + cutin / suberin / pectin / lignin / wax / silica / mucilage
Thick, inelastic, mechanical strength
Tertiary cell wall
Sometimes S3 layer of secondary wall
Xylan + mannan
Thick warty wall; gymnosperm tracheids
Middle Lamella:
  • First layer formed during cytokinesis
  • Cementing layer between adjacent plant cells
  • Main cement = calcium pectate
  • Retting of fibres due to dissolution of middle lamella
  • Ripened fruit becomes soft due to dissolution of calcium pectate
  • Pectinase enzyme dissolves pectin / calcium pectate
  • Totally absent in unicellular plants and all fungi
Primary Wall:
  • Elastic
  • Unipartite / single layer
  • Lignin never found in primary cell wall
  • May contain silica, wax, cutin etc.
  • Growth by intussusception
  • Starch is formed by α-D glucose
Secondary Wall:
  • Thick
  • Inelastic
  • Tripartite: S1 + S2 + S3
  • S2 is thickest layer
  • Developed by apposition
  • Pits common
  • Provides mechanical strength, stiffness and hardness
Wall Deposition Substances:

Table 1: Secondary Wall Deposits

Substance
Site
Function / Point
Cutin
Epidermis; continuous layer = cuticle
Waxy substance
Suberin
Cork cells + endodermis
Makes cell impermeable; checks evaporation; fatty acid mixture
Pectin
Collenchyma
Elasticity
Lignin
Xylem vessels, tracheids, sclerenchyma
Highest rigidity; lignified cells thick-walled and dead
Wax
Floating leaves
Protection
Silica
Deposited on wall; Equisetum
Rough touch
Mucilage
Aloe vera, Hibiscus
Slimy, slippery; helps withstand extreme temperature
Cuticle Thickness:
  • Xerophytes: thick cuticle
  • Mesophytes: thin cuticle
  • Hydrophytes: cuticle absent
Wall Growth:

Table 1: Cell Wall Growth

Type
Meaning
Intussusception
Primary wall stretched and new material inserted
Apposition
Secondary wall materials deposited as thin layers
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CELL TO CELL CONDUCTION SYSTEM

Table 1: Symplasm vs Apoplasm

Feature
Symplasm / Plasmodesmata
Apoplasm / Pits
Nature
Living system
Non-living system
Definition
Living cytoplasmic strands connecting adjacent plant cells
Unthickened wall area where secondary wall is absent
Connection
Links protoplasts into continuous living system
Dead cells interconnected through pits
Modification
Modification of cell membrane
Present in secondary wall
Found in
Only multicellular plants
Cells with secondary wall
Main structures
Plasmodesmata
Pit cavity + pit membrane

Table 2: Types of Pits

Pit Type
Feature
Common Site
Simple pit
Uniform diameter; lacks pit chamber and torus
Angiosperms; companion cells; sieve tubes
Bordered pit
Flask-shaped cavity; has pit chamber and torus
Gymnosperms; vessels; tracheids
Aspirated pit
Non-functional pit; aperture permanently blocked by torus
Gymnosperms
Pit Membrane:
  • Middle lamella + primary wall
  • Mainly primary wall
Torus:
  • Swollen portion of pit membrane
  • Acts as valve
Special Point: Sclerenchyma fibre may have simple pits as well as bordered pits
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CELL WALL CONSTITUENTS AND UNITS
Important Facts:
  • Cellulose = most abundant carbohydrate of biosphere
  • Cotton contains highest amount of cellulose, about 90%
  • Chitin = second most abundant carbohydrate
Cellulose Organization: β-D glucose → cellulose → micelle → microfibril → macrofibril
Structural Units:
  • Micelle
  • Microfibril
  • Macrofibril
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CELL COAT / GLYCOCALYX
Definition: Outermost covering of animal cell because cell wall is absent
Composition:
  • Oligosaccharides
  • Glycoproteins
  • Glycolipids
Important Points:
  • Terminal sugar of oligosaccharide = sialic acid
  • Oligosaccharide layer helps in cell-to-cell recognition
  • Glycocalyx = glycoprotein + glycolipid
  • Glycoprotein + glycolipid help in site recognition
  • Glycocalyx = site of cell recognition
  • Glycocalyx makes cell membrane asymmetric
  • In cell recognition: oligosaccharide > glycoprotein + glycolipid
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PLASMA MEMBRANE
Synonyms:
  • Cell membrane
  • Plasmalemma
Features:
  • Thin
  • Elastic
  • Living
  • Selectively / differentially permeable
Composition:

Table 1: Membrane Components

Component
Forms / Points
Lipid
Phospholipid, glycolipid, cholesterol
Protein
Extrinsic / peripheral = 25%; intrinsic / integral = 75%
Carbohydrate
Glycolipid + glycoprotein
Phospholipid
Framework molecule; amphipathic; bilayered
Myelin sheath
Lipid 75% > protein 25%
Amino acids
Arginine and lysine found in cytoplasmic membrane
Phospholipid:
  • Head = hydrophilic
  • Tail = hydrophobic
  • Amphipathic molecule
  • Shows hydrophilic + hydrophobic properties
  • Always bilayered
Thickness: About 75 Å
Biological Membrane: Plasma membrane + organelle membrane
Membrane Fluidity: Depends mainly on lipids
Membrane Models:

Table 1: Cell Membrane Models

Model
Scientist
Main Point
Trilamellar / Sandwich model
Danielli and Davson
3 layers: protein outside + phospholipid bilayer middle + protein inside; sequence P-L-L-P
Unit membrane model
Robertson
Similar to trilamellar model; protein fibrillar type; sequence P-L-L-P
Fluid Mosaic Model
Singer and Nicolson
Latest and most accepted; protein iceberg in sea of lipid
Fluid Mosaic Model:
  • Based on freeze-etching / freeze-fracture technique
  • Differs by arrangement of proteins
  • Lipids form continuous layer
  • Protein molecules randomly arranged
  • Proteins: extrinsic, tunnel, intrinsic
  • All carrier proteins are intrinsic proteins
  • Lipids and proteins held by hydrophobic attraction
  • Rotational movement of lipid molecule = flip-flop mechanism
Functions:
  • Controls passage of materials in and out of cell with help of Ca2+ and cAMP
  • Exchange of gases like O2 and CO2
  • Bulk transport
  • Active transport needs energy + carrier protein
  • Passive transport needs neither energy nor carrier protein
  • Exception: facilitated diffusion is passive but requires carrier protein
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JUNCTION COMPLEX
Definition: Cell adhesion structures found in multicellular organisms

Table 1: Junction Complex Types

Type
Feature
Plasmodesmata
Living cytoplasmic strands connecting adjacent plant cells
Desmosome
Spot weld between two adjacent animal cells
Tight junction
Water-tight seal between adjacent animal cells
Gap junction
Channels between adjacent animal cells
Interdigitation
Interwoven processes
Terminal bar
Group of junctional complexes attaching adjacent epithelial cells laterally
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MODIFICATIONS OF CELL MEMBRANE

Table 1: Membrane Modifications

Modification
Found In
Feature / Function
Mesosome
Gram-positive bacteria
Infoldings of cell membrane; respiration + cell division
Lomasome
Fungi
Between cell membrane and cell wall; extracellular cell organelle; cell wall proliferation + elongation
Lamellosome
Blue-green algae
Infolding of cell membrane; helps expansion of cell wall
Microvilli
Digestive tract cells
Finger-like outfolds; absorption of liquids
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PROTOPLASM
Definition and Features:
  • Term coined by Purkinje
  • Huxley called it physical basis of life
  • All life processes occur in protoplasm
  • Best material to study protoplasm = slime moulds due to absence of cell wall
  • Highly organised and most active chemical system of nature
  • Maximum energy conversion occurs in protoplasm
  • Living beings differ from non-living by presence of protoplasm
Composition:

Table 1: Protoplasm Composition

Component
Value / Point
Water
75–85%; highest percentage
Major inorganic constituent
Water
Oxygen
62%; highest element by weight
Carbon
20%
Hydrogen
10%
Nitrogen
3%; lowest among major elements
Inorganic:organic ratio
1:9
Sol-Gel State:
  • Protoplasm exists as sol and gel
  • Inner sol = endoplast / plasmasol
  • Outer gel = ectoplast / plasmagel
  • Sol-gel interconversion due to colloidal nature
  • Conversion of plasmasol into plasmagel = physicochemical phenomenon
  • Cyclosis occurs in endoplast region
Cyclosis:

Table 1: Types of Cyclosis

Type
Movement
Examples
Rotation
Movement in one direction around vacuole
Hydrilla, Vallisneria
Circulation
Movement in different directions around vacuole
Tradescantia
Colloidal Nature Effects:
  • Brownian movement
  • Tyndall effect
  • Cyclosis
  • Amoeboid movement
Temperature / Chemicals:
  • Protoplasm coagulates above 60°C
  • Protoplasm coagulates with concentrated acids / bases
  • Enzymes denature at high temperature
  • Enzymes inactivated at very low temperature
  • Optimum temperature required for enzyme function
Deutoplasm: Non-living substance formed by protoplasm during metabolism, e.g., lipid droplets, yolk
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CYTOPLASM
Parts:
  • Cell organelles
  • Cell inclusions
  • Cytosol
Cytosol:
  • Clear optically homogeneous fluid portion after removing organelles and inclusions
  • Also called cytoplasmic matrix / hyaloplasm / ground plasm
  • Ribosome-containing ground substance = ergastoplasm
Cell Inclusions:

Table 1: Cell Inclusions

Type
Examples
Reserve food
Carbohydrates, fats, proteins
Secretory substances
Colouring matter, enzymes, nectar, pigments
Excretory substances
Alkaloids, glucosides, tannins, gum, resins
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CELL ORGANELLES BY MEMBRANE

Table 1: Organelles by Membrane Covering

Category
Organelles
Triple membrane
Transosomes
Double membrane
Plastids, mitochondria
Single membrane
Endoplasmic reticulum, Golgi bodies, vacuoles, lysosome, peroxisome, glyoxysome, sphaerosome, lomasome
Without membrane
Ribosomes, centrioles, cytoskeleton, nucleolus, chromosomes
Transosome:
  • Found in ovarian follicle of bird
  • Takes part in yolk synthesis / vitellogenesis
  • In other animals, vitellogenesis mainly by Golgi bodies + ER + microfilaments
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PLASTIDS
Basic Features:
  • Double-membrane organelles
  • Discovered / named by Haeckel
  • Mainly found in plants and some protists
  • Absent in fungi
  • Involved in formation and storage of carbohydrates
  • Usually 3 types based on pigment: leucoplast, chromoplast, chloroplast
Leucoplast:
Features:
  • Largest plastid
  • Usually found in underground parts
  • Storage of reserve food

Table 1: Types of Leucoplast

Type
Stores
Examples
Amyloplast
Starch
Potato, rice, wheat
Proteinoplast / Aleuronoplast
Protein
Maize
Elaioplast / Oleosome / Lipoplast
Oil / fat
Ricinus / castor seed
Chromoplast:
Features:
  • Second largest plastid
  • Coloured plastid other than green
  • Contains fat-soluble carotenoids
  • Provides colour to fruits, seeds and flowers
Formation:
  • From chloroplast by replacement of chlorophyll by coloured pigment
  • Green tomato → red tomato due to lycopene
  • Green chilli → red chilli due to capsanthin
  • From leucoplast by development of coloured pigment, e.g., carrot
Other Coloured Plastids:

Table 1: Other Plastids

Plastid
Colour
Example
Phaeoplast
Brown
Brown algae
Rhodoplast
Red
Red algae
Chloroplast:
Features:
  • Discovered by Sachs
  • Named chloroplast by Schimper
  • Green plastid
  • Forms carbohydrates
  • Stores starch temporarily
  • Called kitchen of cell, photosynthetic apparatus, autoplast
  • Present in photosynthetic organisms except photosynthetic bacteria and cyanobacteria
  • In photosynthetic bacteria and cyanobacteria, pigments occur in chromatophores / scattered thylakoids
Shapes:

Table 1: Chloroplast Shapes

Shape
Example
Discoid / oval / tablet / biconvex
General
Cup-shaped
Chlamydomonas
Spherical
Chlorella
Spiral / ribbon / scalariform
Spirogyra
Girdle / ring / collar / horse-shoe
Ulothrix
Reticulate / net-like
Oedogonium
Stellate / star-shaped
Zygnema
Discoid
Vaucheria, Chara
Number:
  • Single chloroplast: Chlamydomonas and Chlorella
  • Single chloroplast per cell: Ulothrix
  • Double chloroplast per cell: Zygnema
  • Maximum chloroplasts: mesophyll cells of leaf
Structure:

Table 1: Chloroplast Structure

Part
Feature / Function
Grana
Site of light reaction
Thylakoids
Structural unit of chloroplast
Quantasomes
Functional unit of chloroplast
Stroma lamellae / fret membrane
Connect grana
Stroma
Ground substance
Matrix of stroma
Contains dark reaction / CO2 fixation enzymes
Stroma DNA + ribosome
Semi-autonomous feature
Quantasome:
  • Photosynthetic pigments present in thylakoid membrane at quantasomes
  • One quantasome has about 280 pigments
  • Eukaryotic quantasome: 230 chlorophyll molecules + 50 carotenoids
  • 230 chlorophyll = 160 chlorophyll-a + 70 chlorophyll-b
  • Prokaryotic quantasome: about 50 chlorophyll molecules
Agranal Chloroplast:
  • Common in algae
  • Present in bundle sheath chloroplast of C4 plants
  • If thylakoids are not stacked, called lamellar chloroplast
Blue Green Algae: Lacks chloroplast; photosynthesis site = photosynthetic lamellae
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MITOCHONDRIA
Names:
  • Power house of cell
  • Electric house of cell
  • Cellular furnace
  • ATP mills
History:
  • Discovered by Kolliker in striated muscle of insects
  • Term mitochondria used by Benda
  • Altman called it bioplast
Occurrence:
  • Size: 0.5 to 1 μm
  • Absent in all prokaryotes
  • Absent in anaerobic eukaryotes
  • Present in aerobic eukaryotes except mature mammalian RBC, cancerous cell and sieve tube of phloem
  • Mature RBC obtains energy by glycolysis
Membranes:

Table 1: Mitochondrial Membranes

Part
Feature
Outer membrane
Permeable due to porins
Outer chamber
Contains adenylate kinase
Inner membrane
Less permeable; has cristae / tubuli
Cristae
Site of oxidation-reduction
Inner membrane / cristae
Carries ETS + oxysomes
Oxysomes:
  • Tennis-racket-like particles on inner mitochondrial membrane
  • Functional unit of mitochondria
  • Head = F1 particle
  • F1 contains ATPase
  • ATPase helps final step of ATP synthesis
  • Base = Fo particle
  • Fo has proton channels
  • ETS occurs at Fo / inner membrane
  • Old concept: 2 protons → 1 ATP
  • New accepted concept in note: 3 protons → 1 ATP
Matrix:
  • Contains about 70% of total cell enzymes
  • Krebs cycle enzymes present in matrix
  • Exception: succinic dehydrogenase bound to inner membrane
  • Fatty acid synthesis enzymes and amino acid synthesis enzymes present
  • Exceptions: ATPase and cytochrome oxidase located in inner membrane
  • Matrix rich in Mn
Functions and Special Points:
  • ATP synthesis process = oxidative phosphorylation
  • Oxidative phosphorylation is O2-dependent and light-independent
  • All mitochondria of a cell collectively = chondriome
  • Mitochondria without outer membrane = mitoplast
  • Acristate mitochondria found in spermatid
  • Petite character in yeast due to mitochondrial inheritance
  • Helps in elongation of fatty acid
  • Forms middle piece of sperm
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ENDOPLASMIC RETICULUM
Basic Features:
  • First observed by Porter from liver cells
  • Single-membrane organelle
  • Unit membrane / trilaminar lipoprotein membrane
  • Not visible under light microscope
  • Continues from nuclear membrane to cell membrane
  • ER connection between cells through plasmodesmata = desmotubule
  • Forms more than 50% of eukaryotic cell membranes
  • Occupies about 10% of total cell volume
Names:
  • Working bench of cell
  • Pipeline of cell
  • Endoskeleton
  • Membrane factory
  • Organelle of detoxification
Well Developed In:
  • Pancreas
  • Liver
  • Digestive gland
  • Fibroblast
Poor / Absent In:
  • All prokaryotic cells
  • Mammalian RBC
  • Egg
  • Embryonic cell
  • Monocyte
  • Poorly developed in sperms and meristematic cells
Components:
  • Cisternae = flattened sacs
  • Tubules
  • Vesicles
  • Cisternae are not parallel in ER but parallel in Golgi bodies
RER vs SER:

Table 1: Rough ER vs Smooth ER

Feature
RER
SER
Amount
2/3 of total ER
1/3 of total ER
Ribosomes
80S ribosomes attached by large subunit via ribophorin
No ribosomes
Main structure
Cisternae + few tubules
Network of tubules + vesicles
Origin
Nuclear membrane
RER
Function
Protein synthesis + glycosylation
Lipid metabolism
Special Terms:
  • Broken RER during ultracentrifugation = microsome
  • Basophilic cytoplasm due to RER / ribosome = ergastoplasm
  • Nissl granules = modification of RER / ribosome
  • Nissl granules rich in RNA followed by protein
  • RER without ribosomes = transitional ER
  • SER in retinal epithelial cells = myeloid bodies
  • Annulate lamellae = precursor of ER
  • Special ER in skeletal muscle = sarcoplasmic reticulum
  • Sarcoplasmic reticulum releases Ca2+
  • Ca2+ helps muscle contraction and vesicle fusion
  • Sphaerosomes formed by SER
  • RER + Golgi complex gives rise to lysosome
Functions:
  • Mechanical support due to microtubules; endoskeleton of cell
  • Intracellular and intercellular transport
  • Keeps cell organelles in position
  • Formation of nuclear membrane and cell membrane
  • Formation of vacuoles, lysosomes, glyoxysome, Golgi bodies, exine of pollen grain and zymogen
  • Detoxification of toxic substances
  • SER in liver helps detoxification
  • SER in liver helps glycogenesis and glycogenolysis
  • Major phospholipid synthesized in ER = lecithin / phosphatidylcholine
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GOLGI BODIES
Names:
  • Idiosome
  • Dalton complex
  • Middle man of cell
  • Baker's bodies
  • Dictyosome in plant cell
History:
  • Discovered by Camillo Golgi from nerve cell of cat and barn owl
  • First stained with Sudan III
  • Therefore called Baker's bodies
Absent In:
  • All prokaryotic cells
  • RBCs
  • Sieve tube
  • Sperm of bryophytes and pteridophytes
  • Cells of fungi
Origin and Structure:
  • Formed from vesicles of SER
  • Consists of parallel cisternae, tubules and vesicles
  • New cisternae constantly formed by budding from SER and fusion of vesicles
  • Outside convex side = forming face
  • Microtubules never found in Golgi body
  • Maximum Golgi bodies in rhizoidal cell of Chara
Functions:
  • Secretion and storage of secretions
  • Chemical modification and transport of materials
  • Cell plate formation during cytokinesis
  • Formation of lysosomes
  • Formation of acrosome in sperm
  • Acrosome helps penetration
  • Secretion of mucilage in root tip
  • Mucilage helps lubrication
  • Formation of sialic acid in cell membrane
  • Formation of yolk, melanin granules, hemicellulose and galactose
  • Glycosidation and glycosylation
  • Protein packaging
  • All complex carbohydrates except glycogen are synthesized by Golgi body
Macromolecular Traffic: Golgi apparatus = principal director of macromolecular traffic in cell
Glycosidation and Glycosylation:
  • Lipid + carbohydrate → glycolipid = glycosidation
  • Protein + carbohydrate → glycoprotein = glycosylation
  • Glycosidation and glycosylation start in ER
Common Enzyme Points:

Table 1: ER and Golgi Enzymes

Organelle
Main Enzyme
RER
Peptidyl transferase
Golgi body
Glycosyl transferase
📚
MICROBODIES AND SINGLE MEMBRANE ORGANELLES

Table 1: Microbodies

Organelle
Important Point
Lysosome
Marker enzyme = acid phosphatase
Lysosome
Produced by Golgi bodies; eats vacuoles; associates with ER for excretion
Peroxisome
Photorespiration
Glyoxysome
Important in plant fat metabolism / germinating seeds
Sphaerosome
Plant lysosome
Lomasome
Found in fungi
Smallest Membrane-Bound Organelle: Lysosome
📚
RIBOSOMES
Names:
  • Palade particles
  • Engine of cell
  • Protein factory of cell
  • RNP / ribonucleoprotein particle
Features:
  • Discovered and named by Palade
  • Smallest unmembranous organelle
  • Electron microscopic organelle
  • Most abundant cytoplasmic organelle
  • Made of rRNA + protein
  • rRNA synthesized in nucleolus
  • Protein synthesized in cytoplasm
  • Ribosomes formed inside nucleolus
  • Nucleolus = ribosomal factory
  • Unit = Svedberg unit / S
  • S = sedimentation coefficient
  • 1S = 10^-13 sec
  • Isolated by fractionation and ultracentrifugation
  • Function = protein synthesis
  • Present in prokaryotes and eukaryotes except RBC and sperms
Types:

Table 1: 70S vs 80S Ribosome

Feature
70S Ribosome
80S Ribosome
Found in
Prokaryotes; mitochondria and chloroplast of eukaryotes
Eukaryotic cytoplasm
Subunits
30S + 50S
40S + 60S
rRNA
30S has 1 rRNA; 50S has 2 rRNA
40S has 1 rRNA; 60S has 3 rRNA
Total rRNA
3
4
Total proteins
55
80
Function note
Prokaryotic protein synthesis
Free: non-secretory proteins; RER-bound: secretory/export proteins
Special Points:
  • Mammalian mitochondria have 55S ribosome, not 70S
  • 55S ribosome subunits = 35S + 25S
  • Two ribosomal subunits joined by Mg2+
  • High Mg2+ concentration can combine two ribosomes into dimer
  • 6 to 8 ribosomes attached to mRNA during protein synthesis = polysome / polyribosome / ergasome
  • During protein synthesis, smaller ribosomal subunit attaches to mRNA
  • Ribosomes present inside mitochondria, plastids and nucleus
  • Therefore called organelle within organelle
  • DNA and RNA are observed by X-ray crystallography, not ordinary microscopy
📚
NUCLEUS
Basic Points:
  • Discovered by Robert Brown from orchid root cells
  • Hammerling proved nucleus as control centre using Acetabularia
  • Not considered a cell organelle
  • Largest extracytoplasmic component
  • Controls heredity and cellular activities
Names:
  • Controlling centre of cell
  • Brain of cell
  • Director of cell
  • Apparatus of heredity
Number of Nuclei:

Table 1: Nucleus Number in Cells

Type
Meaning
Examples
Uninucleated
One nucleus; common
Most cells
Binucleated
Two nuclei
Paramecium; secondary mycelium of Ascomycetes and Basidiomycetes
Syncyte
Multinucleated due to fusion of many cells
Bone marrow, striated muscle, Ascaris epidermis
Coenocyte
Multinucleated due to repeated nuclear division without cytoplasmic division
Phycomycete hyphae, Vaucheria, Botrydium, latex cells
Anucleated
Cell without nucleus
Mature sieve tube, mammalian RBC
Exception: Camel and llama RBC are exception among mammalian RBCs
📚
NUCLEAR STRUCTURE
Nuclear Envelope / Karyotheca:
  • Double-layered
  • Lipoproteinaceous
  • Inner nuclear membrane is smooth because ribosomes absent
  • Separates nucleoplasm from cytoplasm
  • Space between two nuclear membranes = perinuclear space
  • Perinuclear space = 50–70 Å
  • Nuclear pore complex is octagonal
  • Gates in pore = annulus
  • Annulus allows selective transport through nuclear pore
Nucleoplasm:
  • Also called nuclear sap, karyoplasm, karyolymph
  • Contains nucleotides and ions like Mn2+ and Mg2+
  • Cyclosis absent because viscosity not constant
Nucleolus:
  • Discovered by Fontana
  • Term given by Bowman
  • Membraneless structure
  • Ribosomal factory of cell
  • Largest part of nucleus
  • Colloidal mass
  • Contains rRNA and non-histone acidic proteins
  • No DNA
  • Shape maintained by Ca2+ and Mg2+
  • Biogenesis at secondary constriction-I of SAT chromosome
Chromatin:
  • Term chromatin given by Flemming
  • Chromosome name given by Waldeyer
  • Made of DNA and histone proteins
  • DNA is acidic and negatively charged
  • Histone is basic and positively charged
  • Ultrastructure appears as beads on a string
📚
CHROMATIN AND CHROMOSOME
Nucleosome:
  • Beaded structure of chromatin
  • Each nucleosome has 8 histone molecules
  • Histone octamer = 2 copies each of H2A, H2B, H3, H4
  • Double-stranded DNA coils around histone octamer
  • Histone core = Nu body
  • Two nucleosomes joined by linker DNA
Chromatin Stain:
  • Feulgen stain = basic fuchsin
  • Feulgen stain reacts with deoxyribose
  • Specific for DNA / chromatin / chromosome
Euchromatin vs Heterochromatin:

Table 1: Euchromatin vs Heterochromatin

Feature
Euchromatin
Heterochromatin
Staining
Light stain
Dark stain
DNA state
Decondensed
Condensed
Gene activity
Active genes
Inactive genes
Replication
Early in S-phase
Late in S-phase
Chromosome Basics:
  • Condensed chromatin = chromosome
  • Meiotic chromosome with beaded structure = chromomere
  • Haploid set of chromosomes = genome
  • Chromosome = physical basis of inheritance
  • DNA = chemical basis of inheritance
  • Chromosomes carry genes; vehicle / bearer of genes
  • Karyotype = morphological features of chromosomes of a species
  • Idiogram = diagrammatic representation of karyotype in descending order
  • Heteropycnosis = differential staining due to differential thickening
  • Saliva test in Olympic games related to X-chromosome / Barr body
Chromosome Number:

Table 1: Chromosome Number Extremes

Category
Organism
Smallest chromosome number
Haplopappus gracilis
Largest chromosome number
Ophioglossum
📚
CHROMOSOME ULTRASTRUCTURE

Table 1: Parts of Chromosome

Part
Meaning / Function
Pellicle
Sheath / covering of chromosome
Matrix
Ground substance
Chromonemata
Coiled threads forming bulk of chromosome
Primary constriction / centromere
Narrow non-stainable region where chromatids attach
Kinetochore
Protein complex on centromere for spindle attachment
Secondary constriction-I
NOR; forms nucleolus; contains rRNA genes
Secondary constriction-II
Contains tRNA genes
Satellite / trabant
Knob-like structure near secondary constriction-I
SAT chromosome
Chromosome bearing satellite
Telomere
Terminal end / sealed end of chromosome
Centromere vs Kinetochore:
  • Centromere = DNA region
  • Kinetochore = assembling protein complex on centromere
  • Centrosome = microtubule-organising centre of animal cell
Types Based on Centromere:

Table 1: Chromosome Types by Centromere Position

Type
Centromere Position
Anaphase Shape / Feature
Acentric
No centromere
No proper spindle attachment
Metacentric
Middle / centre
V-shaped; arms equal
Submetacentric
Submedian / near centre
L-shaped
Acrocentric
Subterminal / near top
J-shaped
Telocentric
Terminal
I-shaped / rod-like; one arm
Holocentric / polycentric / diffuse centric
Whole surface acts as centromere
Example: Luzula
Study Stage:
  • Chromosome shape best studied in anaphase
  • Structure, morphology, size and number best studied in late prophase and metaphase
  • Most appropriate stage for chromosome study = metaphase
  • Centromeric index = ratio of lengths of two chromosome arms
SAT Chromosome:
  • Satellite = knob-like structure near secondary constriction-I
  • Chromosome with satellite = SAT chromosome
  • SAT chromosome with secondary satellite = tandem SAT
  • At least 2 SAT chromosomes per diploid nucleus
📚
OTHER NON-MEMBRANOUS ORGANELLES

Table 1: Non-Membranous Organelles

Organelle
Important Point
Centriole
Related to spindle formation in animal cells
Cytoskeleton
Microtubules + microfilaments + intermediate filaments
Microtubules
Cytoskeletal support; spindle fibres
Microfilaments
Smallest cell component
Intermediate filaments
Cytoskeletal support
Nucleolus
Ribosomal factory; membraneless
Chromosomes
Physical basis of inheritance
📚
SKELETONS OF CELL

Table 1: Cell Skeletons

Type
Structure
Exoskeleton
Cell wall
Endoskeleton
Endoplasmic reticulum
Cytoskeleton
Microtubules, microfilaments, intermediate filaments
📚
SIZE-BASED NOTES

Table 1: Largest and Smallest Components

Category
Answer
Largest cell component
Nucleus
Largest cell organelle in plant
Chloroplast
Largest cell organelle in animal
Mitochondria
Second largest cell organelle in plant
Mitochondria
Smallest cell organelle
Ribosome
Smallest membrane-bound organelle
Lysosome
Smallest cell component
Microfilament
📚
SEMI-AUTONOMOUS ORGANELLES
Examples:
  • Mitochondria
  • Plastids
Features:
  • Contain DNA, RNA and ribosomes
  • DNA is simple, circular and double-stranded
  • Ribosomes are 70S type
  • Replicate independent of nuclear DNA
Plastids:
  • Plastid carrying genetic information = plastidome
Mitochondria:
  • Endosymbionts of cell
  • Prokaryotic cell within eukaryotic cell
  • Cell within cell
  • Divide by binary fission due to own DNA
  • Apoptosis = programmed cell death caused by mitochondrial DNA
Q1.
The catabolic enzymes are found in
Q2.
Thylakoids are associated with
Q3.
"Cells arise from pre-existing cells only reproduction." This theory was given by
Q4.
Who first discovered living cell?
Q5.
Aerobic respiration inside the cell occurs in:
Q6.
Energy is stored in the cell in
Q7.
“Who first observed and named the cell?”:
Q8.
The protein tubulin is found in
Q9.
The extranuclear cell organelles which posses hereditary materials are
Q10.
The site for protein synthesis is:
Q11.
All of the following are membrane bound except
Q12.
Which of the following structure is not possessed by in eukaryotic cell?
Q13.
Enzymes that take part in glycolysis are present in:
Q14.
Which of the following is true for prokaryotic cells?
Q15.
Genetic material of eukaryotic organism is:
Q16.
Which thin delicate membrane surrounds the central vacuole and is in contact with the cytoplasm
Q17.
Which of the following is correct?
Q18.
The layer found between cell wall of two cells is
Q19.
Control centre of the cell is
Q20.
The sugar present in fruits, honey and nectar is:
Q21.
In nucleic acids, nucleotides are joined by:
Q22.
Metabolic event taking place in cell is:
Q23.
Which is not a lipid?
Q24.
The amino acid 'Glycine' is found:
Q25.
All proteins are:
Q26.
Which is true about lipids?
Q27.
Lipids are:
Q28.
Which of the following is correct?
Q29.
All nucleic acids are made of:
Q30.
The aspect of protein structure to which the genes most directly relates:
Q31.
Protein that gives off amino acids only on hydrolysis
Q32.
which one of the following is not a part of nucleotides:
Q33.
Molecular formula of cholorophyll is:
Q34.
As the size of protein increases:
Q35.
X-ray crystallography is used to study:
Q36.
Pyrenoids are found in:
Q37.
Ribosome in prokaryotes is:
Q38.
Large number of grana occurs in:
Q39.
A thin membrane that envelops the structure chromosome is known as:
Q40.
The cell wall is interrupted by minute pores called:
Q41.
Which of the following occurs in mitochondria:
Q42.
The process by which all organells are separated from cell is:
Q43.
Which microscope would you use to examine a culture of living cells?
Q44.
When all organelles are removed from a cell, the remaining fluid like portion left is called:
Q45.
The temperature at which protoplasm coagulates is:
Q46.
Which process occurs in chloroplast?
Q47.
What is wrong about mitochondria?
Q48.
What is wrong about golgi bodies:
Q49.
Chloroplast are green due to presence of:
Q50.
Eukaryotic cells differ from prokaryotic cells in
Q51.
Endoplasmic reticulum is made up of:
Q52.
Which is wrong?
Q53.
Description correctly matching corresponding junction type is:
Q54.
The cell and the corresponding substances stored in storage parenchyma:
Q55.
Chromosomes at anaphase are of various shapes due to position of centromere. It is J-shaped when centromere is at:
Q56.
In histology, the details of tissue structure observed through a definite technique, when involves treatment of tissues in an order. The correct sequence is:
Q57.
A tissue is a group of cells which are:
Q58.
Cell drinking is referred to:
Q59.
Which organelle contains maximum enzyme of cell?
Q60.
Prokaryotic genetic system has:
Q61.
The term "suicidal bag" is used for:
Q62.
Which of the following structure other than nucleus contains DNA?
Q63.
Proteins are synthesized by:
Q64.
A light microscope has a magnification of about:
Q65.
The protein embedded in the lipid in the cell membrane is:
Q66.
Enzymes for various functions are found in complex form in
Q67.
Mitochondria are found in
Q68.
Animal cells inter junctions are called
Q69.
Lysosomes contain enzymes which are:
Q70.
The polypeptide assembly can be found on:
Q71.
Beet roots are coloured due to presence of
Q72.
Lysosomes are called suicidal bags because they have
Q73.
Lipid bilayer is impermeable to
Q74.
Plant cells connected by channels through their walls called:
Q75.
Main component of endoplasmic reticulum is
Q76.
The most prime component of cell wall is
Q77.
Who coined the term cell?
Q78.
Function of golgi bodies is
Q79.
Which of the following lack nucleus?
Q80.
Bacterial and eukaryotic cell have similar
Q81.
Cellular totipotency is demonstrated by
Q82.
Spindle fibre is made up of protein:
Q83.
Core element of chlorophyll is
Q84.
Protein synthesis takes place in:
Q85.
The most common character between prokaryotes and eukaryotes is
Q86.
Glycosylation is carried out by
Q87.
Chromoplasts are modified plastids, rhodoplast is mainly consists of pigment.
Q88.
If 'G' represents gametophytec generation and 's' sporophytic generation in plant, the sequence in their life cycle is
Q89.
Nucleus of cell was discovered by
Q90.
Red colour of tomato is due to
Q91.
The filaments of cilia and flagella are composed of:
Q92.
The point which provides the site for the spindle attachment is called
Q93.
Which of the following is common to prokaryotes and eukaryotes?
Q94.
The layer of the cell wall that is formed soon after the cell division and is composed of calcium pectate is
Q95.
Oxysomes is present in
Q96.
Largest cell organelle is
Q97.
Which one of the following is the largest cell component?
Q98.
ATP synthesis takes place in
Q99.
ATP is formed inside mitochondria at
Q100.
Which one of the following in a cell is non-living?
Q101.
Which one of the following has single membrane structure?
Q102.
Which of the following in a cell is non-living?
Q103.
The prokaryotes have
Q104.
Centromere is a part of
Q105.
Chiasma formation occurs during
Q106.
Which of the following part of a cell is non-living?
Q107.
Nucleolus in plant cell is involved in the synthesis of
📚
ADDITION
Q1.
Thylakoids are associated with...
📅MOE 2059
Q2.
Glycosylation of lipids and proteins is carried out by the...
📅BPKIHS 2016
Q3.
Who coined the term 'Cell'?
📅BPKIHS 2013
Q4.
Who first discovered the living cell?
📅MOE 2059
Q5.
The nucleus of a cell was discovered by...
📅BPKIHS 2014
Q6.
The sugar present in fruits, honey, and nectar is...
📅IOM 2001
Q7.
Which of the following is NOT a lipid?
📅BPKIHS 2002
Q8.
All nucleic acids are made of...
📅BPKIHS 2002
Q9.
Prokaryotes differ from eukaryotes in...
📅IOM 2017
Q10.
Prokaryotic genetic system has...
📅BPKIHS 2006
Q11.
What is common between prokaryotes and eukaryotes?
📅IOM 2014
Q12.
Which microscope would you use to examine a culture of living cells?
📅IOM 1996
Q13.
A light microscope has a magnification of about...
📅BPKIHS 2007
Q14.
X-ray crystallography is used to study the...
📅BPKIHS 2007
Q15.
The control centre of the cell is the...
📅MOE 2055
Q16.
Plant cells are connected with each other by...
📅CEE 2022
Q17.
The main component of the Endoplasmic Reticulum is...
📅BPKIHS 2016
Q18.
Which organelle is known as the 'Engine of the Cell'?
Q19.
Aerobic respiration inside the cell occurs in the...
Q20.
The marker enzyme of mitochondria is...
Q21.
The protein embedded in the lipid bilayer of the cell membrane is called...
Q22.
Lipid bilayer is impermeable to...
Q23.
'Cell drinking' is referred to as...
Q24.
The most prime component of the plant cell wall is...
Q25.
The layer found between the cell walls of two plant cells is called the...
Q26.
Maximum cellulose is found in the...
Q27.
The genetic material of a eukaryotic organism is...
Q28.
Which of the following structures, other than the nucleus, contains DNA?
Q29.
Ribosomes are synthesized in the...
Q30.
Chloroplasts are green due to the presence of...
Q31.
The red colour of a tomato is due to the...
Q32.
The core element of chlorophyll is...
Q33.
Enzymes that take part in glycolysis are present in the...
Q34.
The metabolic event taking place in a cell is best described as...
Q35.
Which process occurs in the chloroplast?
Q36.
Spindle fibre is made up of the protein...
Q37.
The point which provides the site for spindle attachment is called the...
Q38.
The filaments of cilia and flagella are composed of...
Q39.
Who proposed that 'Cells arise from pre-existing cells only by reproduction'?
Q40.
In mitochondria, protons accumulate in the...
Q41.
The largest cell organelle in a plant cell is generally the...
Q42.
Which one of the following in a cell is non-living?
Q43.
Which one of the following has a single membrane structure?
Q44.
Cellular totipotency is demonstrated by...
Q45.
The prokaryotes have...
Q46.
Centrioles are made up of...
Q47.
Cristae are associated with...
Q48.
Which structure in bacteria is responsible for motility?
Q49.
Which organelle is called the 'ribosome factory'?
Q50.
Which of the following is called 'suicide bag'?
Q51.
Which one is known as 'cell sap'?
Q52.
Who discovered the cell?
Q53.
The power house of cell is...
Q54.
The extranuclear cell organelles which possess hereditary materials are...
Q55.
ATP synthesis in mitochondria takes place...
Q56.
Which of the following is a function of the Golgi body?
Q57.
The organelle that helps in cell plate formation is the...
Q58.
The nucleolus has maximum size during...
Q59.
The main component of the middle lamella is...
Q60.
Which of the following is the non-living part of a plant cell?
Q61.
The prokaryotic cell differs from a higher plant cell wall in having...
Q62.
The structure present outer to the plasma membrane but inner to the cell wall is the...
Q63.
The organelle performing a similar function in both plant and animal cells is the...
Q64.
The term 'Microsome' refers to...
Q65.
The simplest amino acid is...
Q66.
β-oxidation of fatty acids occurs in the...
Q67.
The organelle responsible for the origin of plasmodesmata is the...
Q68.
Chromosomes consist of...
Q69.
The branch of biology dealing with the structure and function of the cell is...
Q70.
Who demonstrated totipotency in plant cells?